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KMID : 0377619700180040277
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Korean Jungang Medical Journal
1970 Volume.18 No. 4 p.277 ~ p.286
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Phage Typing of Mycobacterium Tuberculosis Strains Isolated in Korea
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Abstract
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Since 1910, when Shigella phage was first isolated, a number of phages acting on various microorganisms have been reported. The mycobacterial phage has, however, been made a little progress, compared with those of enteric bacilli. The main handicaps in studying mycobacterial phages are the variability of host ranges and the tediousness of the host cell cultivation.
Phage typing has become one of the most valuable tools in determining the epidemiological source of various infectious diseases. Recently W.B. Redmond has reported that a certain mycobacterial phages, DS6A, GS4E, AG1, and. D29, which he isolated, may be used in differentiating Mycobacterium tuberculosis (H37Rv) from other mycobacterial strains.
The author has further investigated the characteristics of these mycobacterial phages and tried to phage type the mycobacterial strains isolated in Korea, employing the phages and techniques recommended by W. B. Redmond10). The results were as follows.
1. A high concentration of mycobacterial phages tend to inhibit the growth of mycobacterial cells without any differentiation, misleading the cleared spot as the product of real cell lysis by the action of specific phages. In determining the RTD of any mycobacterial phages, therefore, it is necessary to check whether the phage suspension is able to produce enough plaques at the concentration in the neighborhood of 5X103 per ml.
2. The standard human strain of Mycobacterium tuberculosis, H37Rv, may be differentiated from other cells by the phage typing technique, since cells of H37Rv were uniquely acted on by all of the four phages while other mycobacterial cells responded to the phage action from total negative to partly positive lysis.
3. However, the phage typing test on the 200 Niacin-positive mycobacterial strains isolated in Chonnam province, Korea, revealed a sharply contrasting phenomenon: only 46 strains responded positive to all of the four phages; strains of roughly two this third were totally negative to any phages; 22 strains responded positive only to AG1 phage; 4 strains reacting positive to DS6A and AG1.
4. An attempt to isolate myobacterial phages has been made Two strains, AH1 and AH2, were obtained. Although most of the properties of these phages are still remained to be investigated, a tentative phage typing test using these isolated phages revealed that not only the standard strain, H37Rv, but also most of the isolated mycobacterial strains were very sensitive to the lysis action of these two phages.
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